Antibiotic-resistant biofilm is a complex microbial community associated in the high risk of morbidity and mortality of hospitalized patients. The current study aims to determine the antibiofilm activity of invasive Erigeron floribundus plant extracts often known as "abas-abas" against Staphylococcus… More

Antibiotic-resistant biofilm is a complex microbial community associated in the high risk of morbidity and mortality of hospitalized patients. The current study aims to determine the antibiofilm activity of invasive Erigeron floribundus plant extracts often known as "abas-abas" against Staphylococcus aureus and Pseudomonas aeruginosa. Bacterial strains were grown in M1 medium supplemented with fructose and cultivated for 24 and 48 hours at 30°C. Higher % biofilm inhibition was observed in gram-positive bacteria Staphylococcus aureus in most of the solvent use in extraction. Qualitative phytochemical screening was also evaluated and revealed the presence of tannins, flavonoid, saponins, steroid and the absence of alkaloid. The existences of these phytochemicals in the plant could be used to generate synthetic medications as a source of precursors. Moreover, this study also reveals that E. floribundus extract had antibiofilm action against the isolated nosocomial bacteria, implying that it could be used as an alternative to prevent microbial biofilm development. Less

With growing reports of multidrug-resistant pathogens causing untreatable human infections, the need for new antimicrobial therapies is becoming increasingly important. This study was conducted to investigate the antimicrobial properties of the leaf extracts of Premna odorata Blanco, Petersianthus quadrialatus Merr.,… More

With growing reports of multidrug-resistant pathogens causing untreatable human infections, the need for new antimicrobial therapies is becoming increasingly important. This study was conducted to investigate the antimicrobial properties of the leaf extracts of Premna odorata Blanco, Petersianthus quadrialatus Merr., Shorea astylosa Foxw., and Tridax procumbens Linn. The medicinal importance of these plants remains understudied despite their abundant distribution and endemism in the Philippines. A disk diffusion assay was utilized to test the antimicrobial properties of T. procumbens leaf extract against Pseudomonas aeruginosa and Staphylococcus aureus. On the one hand, the fungal colony and spore germination assay was used to test P. odorata, P. quadrialatus, and S. astylosa leaf extracts against Aspergillus niger. Different concentrations of leaf extracts were prepared and compared with positive and negative controls. T. procumbens minimally inhibits the growth of P. aeruginosa (10 mm) and S. aureus (10 mm), while S. astylosa leaf extracts revealed the most significant inhibition on colony growth (5.38 mm) and spore germination (15). P. odorata and P. quadrialatus showed the least (28.35 mm; 82.17) and moderate (10.97 mm; 49.5) inhibitory potentials, respectively. The discovery of new antimicrobial compounds from these plant extracts is seen as a potential resolve to the pressing problem of antimicrobial resistance. Less

Identification and subsequent isolation of anti-dengue active compounds in medicinal plants are potentially useful for developing antiviral chemical analogues. This study investigated the potential use of aqueous, methanolic, and ethanolic extracts of Syzygium sp. “O-on” against bacterial co-infection of Staphylococcus… More

Identification and subsequent isolation of anti-dengue active compounds in medicinal plants are potentially useful for developing antiviral chemical analogues. This study investigated the potential use of aqueous, methanolic, and ethanolic extracts of Syzygium sp. “O-on” against bacterial co-infection of Staphylococcus aureus and Pseudomonas aeruginosa in dengue. Taxonomic identification of Syzygium sp. “O-on” collected from Mindanao signifies possible new species through their distribution, ecology and conservation status. Phytochemical analysis showed that crude aqueous and organic solvent extracts contain medicinally important bioactive compound. Highest antimicrobial potentials were observed for the methanolic extracts of Syzygium sp. which inhibited 62.5 and 51.16% of the tested microorganisms, respectively, with higher activity against antibiotic-resistant bacteria (83.3%). We suggest that the Syzygium sp. could be potentially exploited to control the behaviors of this pathogen as well as for its utilization as a lead compound in screening for anti-dengue agents based on new antimicrobial targets. Less