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Azadirachta indica A. Juss., Tinospora rumphii Boerl. and Vitex negundo against Sarcoptes scabiei in vitro

Author(s): Obedencio, Jose Jr M, Daguro, Ted Aries A, Dargantes, Alan P, Abella, Jose Alexander, Montemayor, Maria Lebeña, Batain, Jesalyne Joy Marie B

Published: 24 Feb 2022
Volume: 25 (2021) Issue 2, Page: 6, Category: Natural Science,
DOI: https://doi.org/10.52751/cczy2874


Abstract

The study aimed to evaluate the acaricidal efficacy of the ethanol leaf extracts of Azadirachta indica A. Juss., Tinospora rumphii Boerl., and Vitex negundo against Sarcoptes scabiei var. canis. It also aimed to determine the concentration level of each leaf extract which has the highest acaricidal activity. Ethanolic leaf extracts from the plants were prepared in 10% and 50% concentrations. Mite mortality was not ed as percentage efficacy and was measured 0.5, 1, 2, and 6 hours post-exposure. The study utilized Completed Randomized Design and One-way Analysis of Variance (ANOVA) for the data. All concentration levels of the plant extracts showed acaricidal potential. Activity for 10% and 50% ethanolic extracts of T. rumphii, V. negundo, and A. indica were 73.33% and 93.33%, 63.33% and 93.33%, and 36.67% and 76.67%, respectively. As indicated, T. rumphii showed the best acaricidal activity followed by V. negundo and A. indica. It also showed that only the 50% concentration level of T. rumphii and V. negundo have comparable acaricidal effects with a commercial acaricidal solution. It is recommended that further studies will be conducted to determine the lowest effective concentration level for each leaf extract, its toxicity, its bioactive compounds, and in vivo trials.


Keyword(s): acaricide ethanolic extract sarcoptic mange


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